Your little girl is emotionally upset and needs time as Ana said above, Her whole world is upside down and she did not get to lay and raise a clutch. If she is young the hormones are racing. first of all shorten her light hours and reduce protein until the cere is bluish white. Keep her in a separate room for awhile
put radio or a series of CD's on to reduce isolation experience. Then start bonding and training-really work on loving instead of pushing her away because she is acting out. Finally after your mama hen has finished her clutch and if the hen is acting out has calmed down let the hens only have time together.Since she is never to have a nest the best you can do is work on your relationship and if possible in the future return the second hen into a cage beside and perhaps in time a cage together. Your 4 boys will be fine. It is usually safer to keep hens and cocks in separate aviaries. I have 2 old hens that preen and snuggle and have been in their own flight for years and are happy. The older your birds get the less the hormones will bother them.
The hen who is acting out will become a house companion. She is not stupid
and is trying to let us stupid people know that she needs some help and reassurance. Some thing in this hen's history probably as a chick has set this off. You might try clicker training and talk to Ann Marie/ Wiki. The reward and clicker hopefully will redirect her attention and those treats may soften her heart. She is fighting mad!! So she need to cool off a little first.
Follow Ana's guidance and later start a new process with her. Best wishes, Jo Ann